2024 Vol.7 Dec.N06

• Title: Development and Application of the Automated High-Precision Rapid Hemagglutination Titer Determination System
• Name: Yueying Pan, Yuting Chen, Yuhong Guan, Hui Chen, Yanqi Wu
• Company: Hunan University of Technology,Zhuzhou、412007 China
• Abstract:

  This study presents the development of the HIE-R1001, a high-accuracy, rapid automated hemagglutination (HA) titer determination system. The system leverages computer vision and image processing technologies to automate test readings, thereby eliminating the subjectivity of analysts. In HA tests, the system automatically assigns titer values to each sample and creates an archive of HA reaction plate images as raw data.

  Compared to manual interpretation, the HIE-R1001 achieved a 95.8% concordance rate across 800 cases, with 99.75% of cases differing by no more than one titer value from manual judgments, demonstrating a high degree of consistency. Furthermore, the HIE-R1001 boasts extremely fast interpretation speeds, completing image capture, analysis, result return, and image and result storage in just 3 seconds, significantly enhancing analytical efficiency. This system not only reduces the need for visual inspection and misjudgments due to visual fatigue but also lowers labor costs and minimizes the risk of personnel exposure to hazardous test environments. Additionally, the system's capability to store experimental data and raw images facilitates traceability and enhances the operational speed of large-scale clinical testing.

  
  

• Keyword: Hemagglutination assay, Automation, Image analysis
• DOI: 10.12250/jpciams2024090724
• Citation form: Yueying Pan, Yuting Chen, Yuhong Guan, Hui Chen, Yanqi Wu.Development and Application of the Automated High-Precision Rapid Hemagglutination Titer Determination System[J]. Computer Informatization and Mechanical System,2024,Vol.7,pp.105-107

References

[1] Sadhana S. Kode, Shailesh D. Pawar, Deeksha S. Tare, Jayati Mullick. Application of frozen and stored glutaraldehyde-fixed turkey red blood cells for hemagglutination and hemagglutination inhibition assays for the detection and identification of influenza viruses, Volume 289,2021,114046,

[2]Priyanka Panwar. Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination, Volume 223,2024,106541,

[3] Yijia Zhang, Li Li, Xin Xin, Lifeng Chang, Haowei Luo, Wenna Qiao, Jun Xia, Jihui Ping & Juan Su. (2024) Effects of H9N2 avian influenza virus infection on metabolite content and gene expression in chick DF1 cells. Poultry Science 103:10, pages 104125.

[4] Hutchinson EC. Influenza virus. Trends Microbiol. 2018;26(open in a new window):809–810.

[5]Killian, M.L. (2020). Hemagglutination Assay for Influenza Virus. In: Spackman, E. (eds) Animal Influenza Virus. Methods in Molecular Biology, vol 2123. Humana, New York, NY.

[6]OIE (2018) Manual of diagnostic tests and vaccines for terrestrial animals: avian influenza. World Organization for Animal Health (OIE). http://www.oie.int/standard-setting/terres trial-manual/access-online/. Accessed 14 Jan 2019